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Mass Production System for Target Proteins and Pep-tides(e.g. Antibodies and Diagnostic Medicines)

We provide a system for the mass production of target proteins and peptides (transforming cells, purified proteins and peptides), using (a) the mechanism of biosynthesis, both production and repression (feedback inhibition), that a cultured cell system (e.g. Tobacco BY-II cells) of higher plants possesses, and (b) the plant virus mechanism controlling silencing of the host cells ( e.g. Tobacco BY-II cells).

Since this system makes use of higher plant cells, its products can be cultured using nutrient solutions based on inorganic salt. The system also makes possible post-translational modification of phosphorylation and glycosylation and the construction of protein-folding conformation, which would be difficult with the technologies that make use of microorganisms such as Escherichia coli (E. Coli) and yeasts.

This system produces mass-produced cells from cloned target proteins and peptides.

Examples of the proteins that are produced with the system include
  1. jellyfish-based green fluorescent proteins (GFP)
  2. E. Coli-based dihydrofolate reductase
  3. interferon-gamma
  4. interferon-alpha
  5. CPI-17 protein functional domain gene
  6. single strand antibody gene
  7. calmodulin genes
  8. plant-based lectin
  9. others

Vector

Transcription vector

Trans cription vector

Virus vector

Virus vector

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System

system

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Advantages of the system using transgenic plant cells

  1. Mass-Production of Target Proteins and Peptides
  2. Low Cost for Production(Medium, Incubation Facility)
  3. Production of Eucaryotic-Proteins(Post-Translational Modification, e.g. Phosphorylation and Glycosylation, and Folding
  4. Easy Purification of Target Proteins and Peptides

Expression of Green Fluorescent Protein(GFP) Originating in Jellyfish(Observation under Fluorescence Microscope)

Without Induction

With Induction

Expression of Green Fluorescent Protein(GFP) Originating in Jellyfish

Expression of Green Fluorescent Protein(GFP) Originating

Expression and Physiological Activity of Dihydro Folic Acid Reductase(DHFR)

Expression and Physiological Activity of Dihydro Folic Acid Reductase(DHFR)

Expression of Human Gamma Interferon(hIFNγ) in Transgenic Tobacco Cells
(Detected by Western Blotting)

Expression of Human Gamma Interferon(hIFNγ) in Transgenic Tobacco Cells(Detected by Western Blotting)

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  • Activated Plant Productivity
  • Mass Production System for Target Proteins and Pep-tides(e.g. Antibodies and Diagnostic Medicines)
  • Research and Development of Novel Cross-Breeding Systems Using Gene Maps